芬蘭流感A病毒診斷試劑盒

廣州健侖生物科技有限公司

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芬蘭流感A病毒診斷試劑盒

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【公司名稱】 廣州健侖生物科技有限公司
【市場(chǎng)部】    楊永漢

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【騰訊  】 2042552662
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號(hào)二期2幢101-103室

脫片產(chǎn)生的原因有哪些？
（1）多聚賴氨酸玻片質(zhì)量的問(wèn)題。我原先是買的，邁新按說(shuō)也是不錯(cuò)的，可是都脫成什么樣子了。后面補(bǔ)做第二批時(shí)用的病理科老師自己做的片子，要好一點(diǎn)。
（2）組織切的不好，切片機(jī)的問(wèn)題例如比較老的舊的機(jī)器切的厚或者不均勻，或者切片者手法不好等。
（3）沒烤好，時(shí)間短，溫度不夠之類。
（4）操作的時(shí)候甩的太猛了，有脫片嫌疑的片子不甩或輕輕甩，用衛(wèi)生紙從邊緣上慢慢吸水。
（5）修復(fù)的問(wèn)題：抗原修復(fù)的時(shí)候高壓時(shí)間過(guò)長(zhǎng)了，或者放進(jìn)100度的修復(fù)液時(shí)手法不好，咚的一聲就丟進(jìn)去了，這樣超容易脫片。此外，用EDTA修復(fù)比檸檬酸容易脫片，但是你要用到EDTA的時(shí)候也沒辦法，只有從另外的問(wèn)題上著手。
（6）一旦見到有組織漂起來(lái)操作就更加要謹(jǐn)慎，用PBS的時(shí)候盡量用泡的，不要沖。基本上把這些方面都注意到了，能改善的盡量改善，脫片可以減少很多。
（7）組織的問(wèn)題，我用的組織癌癥的很多，越是癌癥組織有壞死之類越容易脫。

What are the reasons for the off-chip?
(1) Poly-lysine slide quality problems. I was originally bought, Mai Xin said that is also good, but all off into what it looks like. Back to make the second installment of pathology teachers to do their own piece, a little better.
(2) tissue cutting is not good, slicing machine problems such as the older or older machine cut thick or uneven, or slicing is not good practices.
(3) no good roasted, short time, the temperature is not enough and the like.
(4) When operating the rejection too fast, there is a film off the suspect the best not to dumped or gently thrown, with the toilet paper from the edge of the water slowly.
(5) the problem of repair: high pressure antigen repair time is too long, or into the repair fluid at 100 degrees when not good way, the boom thrown into it, so ultra easy to take off. In addition, repairing with EDTA is easier than tabletting with citric acid, but you can not do it with EDTA, just from another question.
(6) Be more cautious once you see organized drifting, try to use bubbles when using PBS, do not rush. Basically put these aspects have noticed, can improve as far as possible to improve, can be reduced a lot.
(7) Organizational issues, I use a lot of tissue cancer, the more the more the cancer tissue necrosis and the like easier to take off.